Journal: Pathology Oncology Research

Article Title: Papillary Endothelial Hyperplasia (Masson Tumor) of the Hand. Surgical and Pathological Consideration from Seven Cases Using New Vascular Markers

doi: 10.1007/s12253-020-00838-8

Figure Lengend Snippet: Summary of patient age, gender, location of lesion, appearance of the lesion, duration of symptoms , previous treatments, radiological examinations, surgical care, postoperative complications, recurrences, pathological markers

Article Snippet: Tissue sections of 3 μm thickness were cut from the blocks, followed by deparaffinization in xylene and retrieval using either the Bond Epitope Retrieval Solution 1 (pH ∼ 6) or the Bond Epitope Retrieval Solution 2 (pH ∼ 9) (Leica Microsystems, Wetzlar, Germany) at 99–100 °C for 20–30 min, and immunostained using a monoclonal mouse anti-ASMA antibody (clone:1A4; 1:400; Agilent DAKO, USA California Santa Clara), monoclonal mouse anti-human ERG (clone EPR 3864; ready to use; Ventana, Roche Diagnostics USA Indianapolis), monoclonal mouse anti-human WT1 (clone 6F- H2; 1:300; BioSB, USA Santa Barbara CA), monoclonal mouse anti-human CD31, (clone JC70A; 1:300; Agilent Dako, USA California Santa Clara) and polyclonal mouse anti-human Glut1 (1:100; Cell Marcque, USA Rocklin California).

Techniques: Biomarker Discovery, Functional Assay

Journal: Pathology Oncology Research

Article Title: Papillary Endothelial Hyperplasia (Masson Tumor) of the Hand. Surgical and Pathological Consideration from Seven Cases Using New Vascular Markers

doi: 10.1007/s12253-020-00838-8

Figure Lengend Snippet: Glut1 is negative in endothelial cells (arrows), only red blood cells are positive (arrowheads) ( a ). WT1 is clearly positive on endothelial cells ( b ), while ASMA displays pericytes ( c ). (Immunohistochemical stain with Glut1, WT1, and ASMA)

Article Snippet: Tissue sections of 3 μm thickness were cut from the blocks, followed by deparaffinization in xylene and retrieval using either the Bond Epitope Retrieval Solution 1 (pH ∼ 6) or the Bond Epitope Retrieval Solution 2 (pH ∼ 9) (Leica Microsystems, Wetzlar, Germany) at 99–100 °C for 20–30 min, and immunostained using a monoclonal mouse anti-ASMA antibody (clone:1A4; 1:400; Agilent DAKO, USA California Santa Clara), monoclonal mouse anti-human ERG (clone EPR 3864; ready to use; Ventana, Roche Diagnostics USA Indianapolis), monoclonal mouse anti-human WT1 (clone 6F- H2; 1:300; BioSB, USA Santa Barbara CA), monoclonal mouse anti-human CD31, (clone JC70A; 1:300; Agilent Dako, USA California Santa Clara) and polyclonal mouse anti-human Glut1 (1:100; Cell Marcque, USA Rocklin California).

Techniques: Immunohistochemical staining, Staining